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Longitudinal effects of aging on serum total and free testosterone levels in. 1 protein purification for binding studies. The apparent free fraction of testosterone was determined by calculating the peak area. Total t level ranging from 8 to 11 nmol/l (2. The validity of finnish national health care registries for identifying disease outcomes has recently been shown [20,21]. Testosterone free, testosterone direct, unbound testosterone. 3 - 14 ng/mg. Epi-testosterone is one of several naturally-occurring testosterone compounds in the body that act as steroids. It is reported that lower tt and free testosterone (ft) levels are associated with hypertension. Free testosterone and testosterone bound to albumin are considered "bioavailable. Oestrogen therapy will usually suppress testosterone levels but often not. Muscle mass and strength losses during aging may be associated with declining levels of serum testosterone (t) in men. 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